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. 2016 Sep 13;3(3):23. doi: 10.3390/vetsci3030023

Figure 2.

Figure 2

Results obtained from all B. bigemina PCR assays examined in this study: (a) Sensitivity of the standard PCR assay determined following agarose gel electrophoresis. M is the Bioline HyperLadder II and the concentrations of B. bigemina DNA are shown above each lane with PCR products estimated at 278 bp; (b) SYBR Green qPCR plots of B. bigemina dilutions with DNA concentrations indicated; (c) TaqMan qPCR plots of B. bigemina dilutions with DNA concentrations indicated; (d) Melting curves from the SYBR Green qPCRs in Figure 2b.