Table 5.
Summary of Babesia bovis genotyping showing BvVAI PCR results compared with ITS alleles determined following capillary electrophoresis and GeneMapper analysis.
| B. bovis Isolates 1 | Isolate Description | BvVA1 1,2 kb |
ITS Alleles 1,2 bp |
|---|---|---|---|
| Dixie | Vaccine isolate | 3.5, 6.8 | 224, 234 |
| H03 | Dixie vaccine following tick passage | 6.8 | 224, 234 |
| H10 | Dixie vaccine following tick passage | 3.5, 6.8 | 224, 233, 234, 243 |
| H92 3 | Field isolate 3 | 5.5, 6.1 | 218, 233, 235, 239, 242, 247 |
| H97 | Field isolate | 6.4, 6.8 | 224, 234, 242 |
| J40 | Field isolate | 6.1, (6.2) | 224, 226, 227, 229, 231, 234, 236, 238, 239, 242 |
| J50 | Field isolate | 6.1 | 224, 233, 234 |
| 32 | Field isolate | 6.2, 8.0 | 224, 234, 237, (309), (337) |
| 33 | Field isolate | Nil | (142), (189), 220, 224, 250, 256, 315, 325, 337, 342 |
| 34 | Field isolate | 6.5 | 218, (224), 234, 244, (302), (337) |
1 Dixie vaccine strain alleles are shown in bold font; 2 Faint bands are indicated by a bracket; 3 No history of Dixie vaccine strain vaccination.