Figure 8.

Active bacterial protein synthesis is not necessary for the assembly of vimentin and SUMO-2/3 moieties at the ApV. A. phagocytophilum infected RF/6A cells were treated with tetracycline or vehicle control. At 5 h, tetracycline was removed to restore bacterial protein synthesis. Cells were collected at 0, 1, 2, and 4 h post-washing and labeled with SUMO-2/3 and vimentin antibodies. The images shown were taken at 0 h post-washing, but are representative of what was observed at all post-wash time points. Host nuclei and bacterial DNA were stained with DAPI. Asterisks (*) denote representative ApVs. Scale bars, 5 μm. Results in all panels are representative of two experiments with similar results.