Skip to main content
. 2016 Sep 21;3(3):25. doi: 10.3390/vetsci3030025

Figure 9.

Figure 9

Pharmacologic inhibition of soluble vimentin reduces the A. phagocytophilum load. (A,B) Treatment of A. phagocytophilum infected cells with WFA does not reduce vimentin assembly around the ApV or alter the bacterial DNA load. A. phagocytophilum infected RF/6A cells were treated with 40 μM WFA for 3 h. Cells were screened with vimentin antibody, stained with DAPI, and analyzed using LSCM (A) or subjected to QPCR analysis using primers targeting A. phagocytophilum 16S rDNA and β-actin (B); (C,D) Treatment with WFA prior to and during A. phagocytophilum infection inhibits vimentin assembly around the ApV and reduces the bacterial DNA load. RF/6A cells were pretreated with 0.5 μM or 1 μM WFA for 2 h and then infected with A. phagocytophilum in the continued presence of WFA. At 24 h, the cells were analyzed by LSCM (C) and QPCR (D). Statistically significant values (*** p < 0.001) are indicated. Results in (A,C) and (B,D) are representative of three and four experiments with similar results, respectively. (A,C) Asterisks (*) denote representative ApVs. Scale bars, 5 μm.