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Relative p16 mRNA expression in 3 feline oral squamous cell carcinoma (FOSCC) cell lines compared to normal cat gingiva. All qRT-PCR was repeated in triplicate using 3 different passages of each cell line. The relative p16 mRNA expression in SCCF1 (n = 3) was significantly greater (55-fold) than in SCCF2 (n = 3), SCCF3 (n = 3), and normal gingiva (** p ≤ 0.0001). p16 mRNA was significantly reduced in the SCCF3 cells compared to normal gingiva (* p = 0.027).
