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. 2016 Nov 17;3(4):34. doi: 10.3390/vetsci3040034

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© 2016 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Images of Rickettsia buchneri ISO7 clone B8 transformed with shuttle vector pRAM18dRGA to express GFP_uv. Transformants were isolated and grown in Ixodes ricinus embryonic cell line IRE11. Panel (A) Island of IRE11 cells containing transformed R. buchneri expressing GFP_uv. Image collected 2.5 months after electroporation and rifampicin selection. Infected cells visualized using a fluorescein isothiocyanate (FITC) filter; Panel (B) Phase contrast microscopic appearance of IRE11 cells heavily infected with transformed R. buchneri. Transformed R. buchneri were in the 23rd serial transfer when imaged. Transformants were maintained in cell cultures at high density (1–5 × 10⁶ cells/mL) and were diluted for this image. Infected cells detach; (C) Same field as shown in panel (B) but visualized using fluorescence microscopy with FITC filter; (D) Composite image made by merging images shown in Panels (B,C). All images taken with a Nikon Diaphot fluorescence microscope. Bar equals 40 µm in all panels.

Images of Rickettsia buchneri ISO7 clone B8 transformed with shuttle vector pRAM18dRGA to express GFP_uv. Transformants were isolated and grown in Ixodes ricinus embryonic cell line IRE11. Panel (A) Island of IRE11 cells containing transformed R. buchneri expressing GFP_uv. Image collected 2.5 months after electroporation and rifampicin selection. Infected cells visualized using a fluorescein isothiocyanate (FITC) filter; Panel (B) Phase contrast microscopic appearance of IRE11 cells heavily infected with transformed R. buchneri. Transformed R. buchneri were in the 23rd serial transfer when imaged. Transformants were maintained in cell cultures at high density (1–5 × 10⁶ cells/mL) and were diluted for this image. Infected cells detach; (C) Same field as shown in panel (B) but visualized using fluorescence microscopy with FITC filter; (D) Composite image made by merging images shown in Panels (B,C). All images taken with a Nikon Diaphot fluorescence microscope. Bar equals 40 µm in all panels.