Figure 2.
Image of Ixodes scapularis cells (ISE6) infected with Rickettsia peacockii expressing the far red fluorescent protein mKATE. Panel (A) Phase contrast microscopic appearance of ISE6 cells infected with transformed R. peacockii. Transformed R. peacockii were in the 23rd serial transfer when image was collected. Transformant is maintained in cell layers seeded at high cell density (1–5 × 106 cells per mL); (B) Same field as shown in panel B but visualized using fluorescence microscopy with TRITC filter; (C) Composite image made by merging images shown in Panels (A,B). All images taken using a Nikon Diaphot fluorescence microscope. Bar equals 40 µm in all panels.