Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Nov 17;3(4):34. doi: 10.3390/vetsci3040034

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Identification of plasmids pRPR and pRAM18d in untransformed R. peacockii (RP) and transformant (T) by PFGE and Southern blot (SB) analysis. Panels A and C. PFGE gels showing prominent chromosomal band and several plasmid bands; Panels B and D. Southern Blots of gels shown in panels A and C; Panel B. Southern Blot probed with digoxygenin labeled pRPR-6 (probe specific RP_p06 gene in pRPR). Arrow points to linear monomer of native 26 kb plasmid of R. peacockii; Panel D. Southern blot probed with digoxygenin labeled GFPuv probe. Note probe binding in lane T only and absence of reactivity in RP lane. Arrows mark putative linear monomer of each plasmid and asterisks indicate their conformational isomers; Panel B. Note pRPR-6 probe reactivity in both RP and T. Linear DNA marker positions are to the left of panel A.