Table 1.
Plasmid constructs used for the transformation of Rickettsia buchneri and Rickettsia peacockii.
| Construct | MW (bp) | Fluoro-Chrome | Antibiotic Selection | Symbiont |
|---|---|---|---|---|
| Shuttle Vector Plasmids | ||||
| pRAM18dRGA | 10,248 | GFPuv | Rifampin | R. buchneri |
| pRAM18dRGA[MCS] | 10,309 | GFPuv | Rifampin | R. buchneri |
| pRAM18dSGA[MCS] | 10,736 | GFPuv | Spectinomycin | R. peacockii |
| pRAM18dSGK(23)[MCS] | 11,525 | GFPuv | Spectinomycin | R. peacockii |
| pRAM18dSFA[MCS] | 10,829 | mKate | Spectinomycin | R. peacockii |
| Himar I Transposase-Transposon Plasmid | ||||
| pCis mCherry-SS HIMAR1 A7 | 8423 | mCherry Spec. | Spectinomycin | R. peacockii |