Table 1.
Viral proteins detected in purified BoHV-1.1 virions.
| BHV-1.1 Protein | Description/Alternative Name | Function | Size (aa) | MW (kDa) | # of Peptides a | # of Spectra a | # Unique Peptides a | % Coverage | emPAI | NCBI Accession |
|---|---|---|---|---|---|---|---|---|---|---|
| Capsid | ||||||||||
| UL6 | Capsid portal protein | DNA encapsidation | 692 | 75 | 4 | 12 | 4 | 6.4 | 0.245 | AFV53413 |
| UL18 | Capsid triplex subunit 2 | Capsid morphogenesis | 316 | 33.2 | 7 | 34 | 7 | 24.1 | 1.637 | AFV53401 |
| UL19 | Major capsid protein | Capsid morphogenesis | 1389 | 150 | 32 | 174 | 32 | 26.0 | 2.073 | AFV53400 |
| UL26 | Capsid scaffolding protein | Scaffold/serine protease | 622 | 63.7 | 2 | 11 | 2 | 4.5 | 0.274 | AFV53392 |
| UL38 | Capsid Triplex Subunit 1 | Capsid morphogenesis | 475 | 50 | 4 | 14 | 4 | 12.8 | 0.374 | AFV53380 |
| Envelope | ||||||||||
| UL1 | Glycoprotein L, gL | Cell entry/cell-to-cell spread | 158 | 17 | 3 | 12 | 3 | 17.7 | 0.874 | AFV53419 |
| UL10 | Glycoprotein M, gM | Virion morphogenesis/membrane fusion | 411 | 42.4 | 5 | 26 | 5 | 11.7 | 1.683 | AFV53409 |
| UL22 | Glycoprotein H, gH | Cell entry/cell-to-cell spread | 842 | 88.3 | 9 | 44 | 9 | 11.3 | 0.688 | AFV53397 |
| UL27 | Glycoprotein B, gB | Cell entry/cell-to-cell spread | 928 | 101.9 | 13 | 83 | 1 | 14.8 | 0.778 | AFV53391 |
| UL44 | Glycoprotein C, gC | Cell attachment | 521 | 55.4 | 13 | 113 | 13 | 29.9 | 3.786 | AFV53374 |
| US4 | Glycoprotein G, gG | Cell-to-cell spread | 444 | 46.6 | 1 | 9 | 1 | 3.4 | 0.116 | AFV53429 |
| US6 | Glycoprotein D, gD | Cell attachment | 417 | 44.9 | 3 | 35 | 3 | 9.4 | 0.551 | AFV53430 |
| US7 | Glycoprotein I, gI | Cell-to-cell spread | 382 | 39.6 | 3 | 12 | 3 | 9.4 | 0.389 | AFV53431 |
| US8 | Glycoprotein E, gE | Cell-to-cell spread | 575 | 61.2 | 3 | 22 | 3 | 7.3 | 0.413 | AFV53432 |
| Tegument | ||||||||||
| UL3.5 | protein V57 | Cell-to-cell spread | 126 | 13.4 | 6 | 77 | 6 | 56.3 | 6.499 | AFV53416 |
| Circ | Myristylated tegument protein | Unknown | 246 | 26.1 | 4 | 14 | 4 | 24.0 | 1.512 | AFV53363 |
| UL16 | Tegument protein UL16 | Possible virion morphogenesis | 343 | 36.4 | 6 | 29 | 6 | 20.1 | 0.823 | AFV53404 |
| UL17 | DNA Packaging Tegument Protein | DNA encapsidation/capsid transport | 703 | 72.6 | 7 | 33 | 7 | 11.7 | 0.455 | AFV53403 |
| UL21 | Tegument protein | Virion morphogenesis | 578 | 60.2 | 5 | 23 | 5 | 9.5 | 0.433 | AFV53398 |
| UL25 | DNA Packaging Tegument Protein | DNA encapsidatoin | 598 | 63 | 3 | 9 | 3 | 7.2 | 0.184 | AFV53394 |
| UL31 | Nuclear egress lamina protein | Nuclear egress | 376 | 40.9 | 1 | 2 | 1 | 2.1 | 0.116 | AFV53387 |
| UL36 | Large tegument protein | Capsid transport | 3291 | 336 | 10 | 47 | 10 | 3.4 | 0.147 | AFV53382 |
| UL37 | Tegument protein | Virion morphogenesis | 1034 | 106 | 7 | 22 | 7 | 8.1 | 0.254 | AFV53381 |
| UL49 | Tegument protein VP22 | Virion morphogenesis | 258 | 26.8 | 8 | 106 | 8 | 43.0 | 6.356 | AFV53370 |
| US1.67 | Unknown | 243 | 27.1 | 4 | 16 | 4 | 15.6 | 1.154 | AFV53426 | |
| Tegument (enzymatic and regulatory proteins) | ||||||||||
| bICP4 | IE transactivator protein | Transcriptional regulator | 1386 | 141 | 2 | 2 | 2 | 1.7 | 0.032 | AFV53424 |
| UL41 | Virion host shutoff protein, Vhs | Cellular mRNA degradation | 459 | 50 | 5 | 28 | 5 | 11.3 | 0.557 | AFV53377 |
| UL42 | DNA polymerase processivity subunit | DNA replication | 408 | 42.6 | 3 | 11 | 3 | 8.1 | 0.28 | AFV53376 |
| UL46 | Tegument protein VP11/12 | Possible gene regulation | 748 | 78.6 | 11 | 86 | 11 | 22.9 | 1.572 | AFV53373 |
| UL47 | Tegument protein VP8 | Possible gene regulation | 741 | 80.5 | 13 | 132 | 13 | 18.2 | 1.88 | AFV53372 |
| UL48 | Trans-inducing factor bTIF/VP16 | Gene regulation/virion morphogenesis | 507 | 53.1 | 6 | 42 | 6 | 10.1 | 1.154 | AFV53371 |
| UL54 | Multifunctional regulator, bICP27 | Gene regulation; RNA metobolism | 400 | 43.3 | 1 | 3 | 1 | 2.3 | 0.11 | AFV53364 |
| US3 | US3 kinase | serine/threonine kinase | 468 | 50.2 | 1 | 3 | 1 | 3.8 | 0.101 | AFV53428 |
BoHV-1.1, Bovine herpesvirus type 1.1; MW, molecular weight, emPAI, exponentially modified protein abundance index; NCBI, National Center for Biotechnology Information; a across biological replicates.