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Primers used for qPCR.
| Target gene | Sequence (5′-3′) Forward Reverse |
Amplicon size (bp) | Annealing temperature qPCR (°C) | Reference |
|---|---|---|---|---|
| tet(O) | ACGGARAGTTTATTGTATACC TGGCGTATCTATAATGTTGAC |
171 | 50 | (1) |
| tet(W) | GAGAGCCTGCTATATGCCAGC GGGCGTATCCACAATGTTAAC |
168 | 60 | (1) |
| rRNA 16S | AAACTCAAAKGAATTGACGG CTCACRRCACGAGCTGAC |
180 | 60 | (2) |
| IntI1 | CGAACGAGTGGCGGAGGGTG TACCCGAGAGCTTGGCACCCA |
312 | 59 | (9) |
| sul1 | CGCACCGGAAACATCGCTGCAC TGAAGTTCCGCCGCAAGGCTCG |
163 | 55 | (25) |
