Figure 3. Translin KO mice display increased levels of several microRNAs targeting the activin A receptor type 1C (ACVR1C) 30 min following training in the object location memory task.

Real-time RT-PCR was performed on hippocampal samples to validate candidate microRNAs. (A) Left, no significant difference was observed between translin KO and WT hippocampus in homecage condition. Right, the levels of let-7c-5p (t-test, p=0.003), miR-125b-5p (t-test, p=0.04), miR-128–3p (t-test, p=0.001) and miR-9–3p (t-test, p=0.03) were increased 30 min after training in translin KO hippocampus relative to WT hippocampus. (B) Venn diagram shows 13 overlapping targets of Let-7, miR-128 and miR-125. The 13 target genes are listed in a descending order of prediction context scores. ACVR1C has the highest context score. * indicates p<0.05, ** indicates p<0.005. Data are represented as mean ±SEM.