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. 2017 Sep 20;6:e27872. doi: 10.7554/eLife.27872

Figure 6. Inhibition of ACVR1C impairs synaptic tagging and long-term memory in WT mice, not translin KO mice.

(A) In hippocampal slices from WT mice, treatment with SB431542 (50 µM), an inhibitor of ACVR1C, starting 10 min before S2 stimulation blocked persistent potentiation in pathway S2 (A2; two-way repeated-measures ANOVA, F(1,8) = 13.9, p=0.007), without affecting 4-train long-lasting potentiation in pathway S1 (A1; two-way repeated-measures ANOVA, F(1,8) = 0.3, p=0.6). Treatment with SB431542 during the last 1 hr of recording impaired the maintenance of S2 potentiation (A4; two-way repeated-measures ANOVA, F(1,8) = 6.1, p=0.039), but not 4-train long-lasting potentiation in pathway S1 (A3; two-way repeated-measures ANOVA, F(1,8) = 1.4, p=0.3). (B1) SB431542 or vehicle was bilaterally injected into the hippocampus immediately after training in the object-location memory task. (B2) While WT mice injected with SB431542 (1 µM) explored the DO significantly less than the vehicle-treated group, translin KO mice injected with SB431542 explored the DO at a level similar to the vehicle-treated group 24 hr after training (two-way ANOVA: genotype, F(1,31) = 9, p=0.005; treatment, F(1,31) = 18.9, p=0.0001; genotype X treatment, F(1,31) = 16.4, p=0.0003; Tukey’s post-hoc, WTvehicle vs. WTSB431542: p<0.0001, KOvehicle vs. KOSB431542: p=0.9). n, number of mice. * indicates p<0.05, *** indicates p<0.00005. Data are represented as mean ±SEM.

Figure 6.

Figure 6—figure supplement 1. Related to Figure 6.

Figure 6—figure supplement 1.

Exploratory behavior during object-location memory task is unaltered in mice injected with SB431542 into the hippocampus. WT mice subject to post-training intrahippocampal infusion of SB431542 or vehicle exhibited similar exploratory behavior across training sessions (A; two-way repeated measures ANOVA, F(1,18) = 0.039, p=0.8) and during testing session (B; t-test, p=0.7). Translin KO mice injected with SB431542 or vehicle after training showed similar total object exploration time across training sessions (C, two-way repeated measures ANOVA, F(1,13) = 0.03, p=0.9), and during testing (D, t-test, p=0.5). n, number of mice. Data are represented as mean ±SEM.