(
A) Experimental timeline and representative pictures showing the expression of CoChR and DREADDi in the injection (LH) and recording sites (LHb). (
B) Traces and time-course plots of LH opto-evoked currents before (
a) and after (
b) CNO application in a LHb neuron recorded in acute slices. Bar graph and plots reporting the overall decrease of the opto-evoked excitatory post-synaptic currents (oEPSCs) upon CNO application (N/n = 3/6 paired t-test, t = 3.97, *p=0.01). (
C) Sample traces and averaged graph showing the effect of LH terminals DREADDi activation on oEPSCs train in LHb neurons (two way ANOVA RM, interaction: F = 3.65, *p=0.01). (
D) Schematics of the double-barrel system allowing a local infusion of CNO in close proximity of the recording site. The time-course graph reports the effect of DREADDi activation on the firing activity of LHb neurons in mice expressing DREADDi in mVTA (N/n = 4/7, one way ANOVA RM, F = 0.57, p=0.57) or LH (N/n = 5/7, one way ANOVA RM, F = 0.26, p=0.72). N = mice, n = cells. 3V, third ventricle, MHb, medial habenula, EPN, entopeduncular nucleus, VmH, ventral medial hypothalamus. See
Figure 2—figure supplement 2—source data 1.