(
A) Timeline for Cre-dependent DREADDi strategy within the VTA. Representative image for DREADDi expression (red) in mVTA somata and terminals in LHb. Sample histograms for Fs-evoked latency to escape over 30 trials in a CTRL and DREADDi-expressing mouse. Bottom. Bar graphs and scatter plots for failure rates and latencies to escape (CTRL vs DREADDi, N = 7 vs 10; unpaired t-test; Failures: t = 1.17, p=0.25; Latency: t = 1.26, p=0.22). (
B) Timeline for Cre-dependent DREADDi expression in LH→LHb. Bar graphs and scatter plots for data collected in the open field (CTRL vs DREADDi; N = 6 vs 6; unpaired t-test, t = 0.85; p=0.41) and in the hot-plate test (N = 7 vs 7; unpaired t-test, t = 0.16; p=0.87). N = mice. MHb, medial habenula, VTA, ventral tegmental area, IPN, interpeducular nucleus. See also
Figure 4. See
Figure 4—figure supplement 1—source data 1.