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. 2017 Sep 20;12(9):e0183774. doi: 10.1371/journal.pone.0183774

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© 2017 Trimpert et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — (A) Ndfip1, Ndfip2, Nedd4 and Nedd4L mRNA expression along the nephron. mRNA was isolated from different mouse nephron segments, and subjected to RT-qPCR to determine Ndfip1, Ndfip2, Nedd4 and Nedd4L mRNA levels. The determined mRNA levels were normalized to that of the housekeeping gene Rpl26 and presented as fraction of the segment with the highest level of expression, which was set to 1. Values are means ± SE from 6 mice. Segments indicated are proximal convoluted (S1) and straight (S3) tubule (PCT, PST), medullary (mTAL) and cortical (cTAL) thick ascending limb of Henle’s loop, distal convoluted tubule (DCT), connecting tubule (CNT), and the cortical (CCD) and outer medullary (OMCD) collecting duct. (B) NDFIP1, but not NDFIP2, co-localizes with AQP2. Cryo-sections of mouse kidneys were subjected to immunohistochemistry for NDFIP1 or NDFIP2 (green), AQP2 (red), and the nuclear dye DAPI (blue). Especially in the cortex and inner medulla, NDFIP1 colocalized with AQP2 in collecting duct principal cells. For NDFIP2, co-localization with AQP2 was never observed. Scale bars represent 25 μm.