Fig. 6.

Inhibiting the SPL enzyme increases S1P levels and has protective effects in the STHdh cell line model of HD. a Untargeted metabolomic measurements show a significant increase in the S1P levels after treatment with DOP (p = 5.03e-5, 14 replicates for untreated samples and 15 replicates for DOP treated samples). b, c SPL inhibition by DOP significantly decreases apoptosis in STHdhQ111 cells. The fold-change of decrease in cell death is significantly higher in STHdh Q111 cells compared to STHdh Q7 cells. Calcein (green) stains viable cells, while propidium iodide (PI—red) is a marker for late apoptotic cells. Scale bar=10 μm. The bar plots show the average levels and the error bar represents the standard deviation in the data. Three independent experiments were performed, with at least 7 replicates each. Two-tailed t-test are used for calculating the p-values