Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 20;8:631. doi: 10.1038/s41467-017-00662-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — The greatly improved detection of acidic N-glycans after enrichment on the TiO₂-PGC chip. In a, the [M+3H]³⁺ ions of an acidic N-glycan were overlapped with the isotopic ions of co-eluted neutral N-glycans and could not be assigned accurately. Using the TiO₂-PGC chip, the acidic N-glycans were enriched efficiently and detected with an enhanced S/N and improved mass accuracy because of the removal of interference from neutral N-glycans. In b, the signal intensity as well as the S/N of acidic N-glycans were significantly enhanced after on-chip enrichment because of the reduced ion-suppression derived from neutral N-glycans/matrix. In c, with the removal of noise derived from neutral N-glycans/matrix, the acidic N-glycan mass accuracy was significantly enhanced, thus providing reliable evidence for the identification of acidic N-glycans. The number in the bracket beside each mass value indicates the mass error (p.p.m.)