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Insertion of Pf3 (A) or KcsA (B) in DOPC liposomes in the absence or presence of YidC, measured by FCS. The proteins were purified and labeled with Atto520, then added to DOPC liposomes (black) or to YidC proteoliposomes (red). The fluorescence was measured for 10 min in the presence of 100 mM KI. Whereas the insertion of KcsA was independent of the YidC insertase, the Pf3 coat protein strictly requires YidC.
