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. 2017 Jun 16;113(6):1342–1352. doi: 10.1016/j.bpj.2017.05.032

Figure 4.

Figure 4

VPS4-dependent release of miRNA and GPI-labeled vesicles. (A) qRT-PCR analysis of 100k EVs from cells expressing VPS4EQ. Values were normalized to spike-in cel-miR-39 RNA and compare mi-RNA in EVs released by control uninduced or VPS4EQ-expressing HEK293 cells. Data are expressed as the mean ± SE of three independent experiments. (B) Confocal image of VPS4EQ-inducible HEK293 cells stably expressing mCherry-GPI. Scale bar, 10 μm. (C) Immunoblot of cell lysate and 100k EVs from HEK293 cells expressing mCherry-GPI with or without VPS4EQ. (D) Quantitation of mCherry-GPI in EVs from three independent experiments, comparing mCherry-GPI release from cells with or without induction of VPS4EQ. The line indicates mean. (E) Quantification of mCherry-GPI particles released from cells comparing control uninduced to VPS4BEQ-expressing HEK293 cells. Values are average particle counts from five fields. Error bars indicate the standard deviation. Data are from one of two biological replicates.