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Nocodazole selectively decreases CD63 release. (A) Serum-triggered EVs released from HEK293 cells treated with either 12 μM nocodazole or dimethylsulfoxide (DMSO) were harvested by PEG/ConA precipitation and immunoblotted for CD63 and CD9. (B) Quantitation of three independent experiments, with the line indicating the mean. Values show the percent of protein in PEG/ConA EVs from nocodazole treated cells compared to EVs from control untreated cells. The lines indicate mean.
