Figure 4. Mitochondrial heme and Fe/S cluster biogenesis are co-regulated.
Mfrn1gt/gt ES cells expressing various alas2 constructs. The schematic in (A) highlights the features of the ALAS2 constructs as well as the anticipated results. (B) PPIX levels were analyzed per milligram of total protein in Mfrn1 gt/gt cells transduced with various lentiviruses, shown in (A). Reprinted with permission from Chung et al 39 and The Journal of Biological Chemistry (The American Society for Biochemistry and Molecular Biology, Inc).