Figure 3.

In Vitro Sensitivities of Infected Human Tumor Cells to 5-FC

Combination of oncolytic and prodrug activation cytotoxicity. (A and B) LoVo (A) and A549 (B) human tumor cells were infected with the CPXwt and CPXtk⁻/gfp::fcu1 at a MOI of 0.01 on LoVo cells and at a MOI of 0.000001 on A549 cells. After 48 hr, cells were grown in the presence of increasing concentrations of 5-FC. Cell survival was determined 3 days later, as described in the Materials and Methods section. Cell viability results are expressed as the percentage of viable cells relative to untreated/non-infected cells. Values are represented as mean ± SD of three individual determinations.