Table 2. The presence of specific DID fluorescence in urethras from study group after manual alignment to the control in IVIS®.
| Goat number | urethra slice number | ||||||
|---|---|---|---|---|---|---|---|
| I | II | III | IV | V | VI | VII | |
| G20 PBS | 0 | 0 | 0 | 0 | 0 | 0 | NS |
| G27 PBS | 0 | 0 | 0 | 0 | 0 | 0 | NS |
| G29 PBS | 0 | 0 | 0 | 0 | 0 | 0 | NS |
| G12 | 0 | 0 | 1 | 1 | 0 | 0 | NS |
| G13 | 0 | 0 | 1 | 1 | 0 | 0 | NS |
| G21 | 0 | 1 | 1 | 1 | 1 | 1 | NS |
| G22 | 1 | 1 | 1 | 1 | 1 | 0 | NS |
| G23 | 1 | 1 | 1 | 1 | 1 | NS | NS |
| G28 | 1 | 1 | 1 | 1 | 1 | 0 | NS |
| G30 | 0 | 1 | 1 | 0 | 0 | 1 | 1 |
| G33 | 0 | 1 | 1 | 1 | 1 | 0 | NS |
| G35 | 0 | 1 | 1 | 1 | 1 | 1 | NS |
„1" indicates the presence and „0'' lack of registered fluorescence signal. Urethra slices in which fluorescence is reported are shaded orange. Heavy gridlines indicates differences between outcome from IVIS® automatic spectral unmixing algorithm application and manual ranges setting. NS—no slice with this number.