Table 5. Settings difficulties for PKH26.
goat number | G35 | unmix | different manual settings | alignment to control G29 | alignment to control G20 | alignment to control G11 | ||
---|---|---|---|---|---|---|---|---|
urethra slice number | I | 1 | 1 | 0 | 0 | 1 | 0 | 1 |
II | 1 | 0 | 0 | 0 | 1 | 0 | 1 | |
III | 1 | 1 | 0 | 0 | 1 | 0 | 1 | |
IV | 1 | 1 | 1 | 0 | 1 | 0 | 1 | |
V | 1 | 1 | 0 | 0 | 1 | 0 | 1 | |
VI | 1 | 1 | 0 | 0 | 1 | 0 | 1 |
Comparison of different IVIS® settings application influence on the fluorescence visible to the recipients eye on the example of the G35 urethra. „1" indicates visible signal, „0” the lack of a visible signal. Urethra slices in which fluorescence is reported are shaded orange.