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. 2017 Sep 20;12(9):e0184976. doi: 10.1371/journal.pone.0184976

Fig 4. Effects of consumption of L. paracasei on ILCs and T effector cells.

Fig 4

(A). gating strategy for FACS analysis of mouse lung. ILC1 were gated on CD45+CD3-CD5-RORγt-NKp46+, ILC2 were gated on CD45+CD3-CD5-RORγt-GATA-3hi cells and ILC3 were gated on CD45+CD3-CD5-RORγt+ cells. The analysis of ILC3 subsets were supported by the expression of NKp46 and CCR6. Numbers adjacent to boxed areas indicate relative percentage of gated populations. Representative results from 4 independent experiments. (B-C) Representative histograms show the absolute numbers of ILC subsets (B) T cells (C) in lung. (D) Lung cells were stimulated for 4h with IL-12, IL-18, IL-33, IL-23 and IL-1β and IFN-γ, IL-5, IL-13 and IL-22 expression in ILCs and T cells was assessed by intracellular cytokine staining and flow cytometry. Graph represents the absolute numbers of cells. Representative results from 4 independent experiments (n = 9/group). *, P<0.05.