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. 2017 Sep 20;12(9):e0184953. doi: 10.1371/journal.pone.0184953

Fig 8. Detection of bUAS transcripts and UDP-apiose.

Fig 8

In vivo indication for the functional activity of bUAS genes and enzymes. (A) RT-PCR analyses showing bUAS transcripts of G. roseus and X. pisi. (B) HILIC-LC-ESI-MS/MS analysis of aq-methanolic (MeOH:chloroform:H2O; 40:40:20, v/v/v) extracts. Negative mode [M-H]- ions diagnostic for UDP-pentose (m/z 535.0, solid line; amplified by a factor of 10), UDP-hexuronic acid (m/z 579.0, dashed line) and Park’s nucleotide (m/z 595.6, dotted line) are displayed.