Table 2. Microsatellite loci amplified in Aedes aegypti.
Locus | Sequences 5’-3’ | Repetitive motif | T (°C) | Size range (bp) | References |
---|---|---|---|---|---|
A10 | F: AATCGTGACGCGTCTTTTG | CT | 60 | 232–242*(232–239) | Chambers et al. [24] |
R: TAACTGCATCGAGGGAAACC | |||||
B07 | F: CAAACAACGAACTGCTCACG | GA | 60 | 100–272*(157–183) | Chambers et al. [24] |
R: TCGCAATTTCAACAGGTAGG | |||||
AT1 | F: CGTCGACGTTATCTCCTTGTT | AT | 55 | 134–170*156–174) | Slotman et al. [25] |
R: GGACCGGAAAGACACAGACA | |||||
AG7 | F: CGTGCGAGTGAATGAGAGAC | GA | 55 | 112–190*(153–185) | Slotman et al. [25] |
R: CATCCTCTCATCAGCTTCTAATAAA | |||||
AG2 | F: TCCCCTTTCAAACCTAATGG | AG | 55 | 96–152*(115–178) | Slotman et al. [25] |
R: TTTGCCCTCGTATGCTCTCT | |||||
AG5 | F: TGATCTTGAGAAGGCATCCA | AG | 55 | 140–164*(170–180) | Slotman et al. [25] |
R: CGTTATCCTTTCATCACTTGTTTG | |||||
AC7 | F: TCGGCAAATTACCACAAACA | CA | 55 | 106–130*(129–143) | Slotman et al. [25] |
R: CATTGGACTCGCTATAACACACA | |||||
AC5 | F: TGGATTGTTCTTAACAAACACGAT | CA | 55 | 104–156*(149–163) | Slotman et al. [25] |
R: CGATCTCACTACGGGTTTCG | |||||
AC1 | F: TCCGGTGGGTTAAGGATAGA | CA | 55 | 140–196*(193–209) | Slotman et al. [25] |
R: ACTTCACGCTCCAGCAATCT | |||||
AG1 | F: AATCCCCACACAAACACACC | AG | 55 | 90–106*(113–129) | Slotman et al. [25] |
R: GGCCGTGGTGTTACTCTCTC |
T = annealing temperature, bp = base pairs
*Values found in the Aedes aegypti tested. In parentheses, size range found in the original study.