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. 2017 Aug 7;113(6):1301–1310. doi: 10.1016/j.bpj.2017.06.061

Figure 2.

Figure 2

Comparison of the effects induced by Chol depletion versus addition of peptides MPER(671–693) or MPER(671–693)L679I on the membrane lateral packing as determined by two-photon excitation GP images of Laurdan-labeled VL GUVs. Laurdan GP imaging of single GUVs (A) and average GP values were determined in the different samples (B). GP value distributions were determined for at least 30 single vesicles, and mean values were depicted as box-and-whisker plots (the ends of the whiskers represent SDs). The mole percents in the lipid mixtures were as follows: POPC/SPM/CHOL/DHSM/POPE/pl-PE/POPS (14:12:46:5:3:13:7) (VL), POPC/SPM/CHOL/DHSM/POPE/pl-PE/POPS (18:16:30:6:4:17:9) (VL-30 mol % Chol), and POPC/SPM/CHOL/DHSM/POPE/pl-PE/POPS (22:18:15:8:5:20:12) (VL-15 mol % Chol). Peptide concentration was 20 nM. Mann-Whitney test: ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05, not significant (n.s.) ≥ 0.05. To see this figure in color, go online.