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The UV spectra of PBDE hapten, protein and conjugates: (a) with BSA and (b) with OVA; absorbance value at the characteristic peak, 292 nm: ODBSA-PBDE = 1.860, ODPBDE-hapten = 1.893, ODBSA = 0.074; 272 nm: ODOVA-PBDE = 1.767, ODPBDE-hapten = 1.893, ODOVA = 0.401; CBSA: 0.20 g/L, COVA: 0.20 g/L, and Chapten: 0.05 g/L; protein and conjugate were dissolved in PBS buffer; hapten was dissolved in DMSO.
