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. 2017 Sep 20;7:11965. doi: 10.1038/s41598-017-12353-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Abnormal fusions between the Dorsal Aorta (DA) and Cardinal Vein (CV) in Ncx1 ^−/− mutants. Two-photon images of Control (A–D) and Ncx1 ^−/− mutant (E–H) embryos at 15–17ss (E9.0) stained with PECAM-1. (A,E) Lateral projection view of the DA and CV. (B–D,F–H) Optical cross sections taken at indicated positions. Red arrow, DA; Blue arrowhead, CV; Yellow arrowhead, DA-CV fusion. Scale bar: 100 um. n = 3 embryos per genotype were analyzed.