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. 2017 Sep 20;7:11965. doi: 10.1038/s41598-017-12353-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DA ECs lacked expression of the arterial marker ephrin-B2 in Ncx1 ^−/− mutants. (A–H) EfnB2GFP expression at 15–17ss in ECs of control (A,B,E,F) and Ncx1 ^−/− mutants (C,D,G,H) in the DA (A–D) and CV (E–H). Red, PECAM-1; Green, EfnB2GFP. Scale bar: 25 um. (I) Proportion of EfnB2GFP positive ECs in the DA and CV. (J) Average EfnB2GFP intensity per EC. ***P < 0.001. Average values ± s.d. are shown; n = 4 embryos per genotype.