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. 2017 Sep 20;7:11965. doi: 10.1038/s41598-017-12353-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DA ECs in Ncx1 ^−/− mutants also lacked expression of a second arterial marker, Connexin-40. (A–H) Cx40 expression at 15–17ss in ECs of control (A–B,E–F) and Ncx1 ^−/− mutants (C,D,G,H) in the DA (A–D) and CV (E–H). Green, PECAM-1; Red, Cx40. Scale bar: 25 um. (I) Average Cx40 intensity (in PECAM1 positive area) in the DA and CV. ***P < 0.001. Average values ± s.d. are shown; n ≥ 4 embryos per genotype.