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. 2017 Sep 20;7:11962. doi: 10.1038/s41598-017-12342-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of BRD4 depletion on expression of genes related to insulin sensitivity in 3T3-L1 adipocytes. Control or Brd4 shRNA-expressing 3T3-L1 cells were treated with medium for differentiation and real-time RT-PCR analyses for Albp, Glut4, Fas, Accα, Accβ, Dgat1, Lpl, Hsl, Aco, Pparγ1, Pparγ2, Creb, C/ebpα, C/ebpβ, C/ebpδ, C/ebpγ, C/ebpζ, Chrebp, Pgc1α, Srebp1, Srebp1α, Lxrα and Lxrβ were performed at 2 and 8 days after differentiation stimulation. The data shown are means ± SEM of 6 wells per condition in a single experiment. *P < 0.05, **P < 0.01 versus the corresponding control cells by Student’s t-test.