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. 2017 Sep 20;7:11942. doi: 10.1038/s41598-017-12219-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Agropyron cristatum chromosome 6P-specific EST-STS marker screening in a wheat background. (a) GISH detection showed 21 pairs of wheat chromosomes and one pair of 6P chromosomes in addition line 4844-12. The arrowheads indicate the addition of chromosome 6P by the green fluorescent signal. (b) The amplification product of EST-STS markers was run on 8% non-denaturing polyacrylamide gel. Agropyron cristatum and addition line 4844-12 are positive controls, and wheat varieties Fukuho and Chinese Spring are negative controls.