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. 2017 Sep 20;7:12016. doi: 10.1038/s41598-017-12191-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HOTAIR enhanced the migration of RCC cells in vitro (A,B) HOTAIR expression in ACHN and A498 cells transduced with a HOTAIR-overexpressing (HOTAIR) or empty vector (EV), determined by real-time PCR. (C) HOTAIR expression levels in HOTAIR-targeted siRNA-transfected Caki-1 cells, determined by real-time PCR. (D,E, and F) Cell-proliferation CCK-8 assays in the indicated cell lines. (G,H, and I) Two-chamber assays using HOTAIR-overexpressing (G,H) or knockdown cells (I) in arbitrary units (a.u.). (J to L) Wound-healing assays. *P < 0.05, Mann-Whitney U test.