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. 2017 Sep 20;7:12028. doi: 10.1038/s41598-017-11086-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Regeneration of motor axons increased by NH treatment. (A) White matter of the spinal cord immunostained against ChAT (red), GAP43 (purple), and counterstained with DAPI (blue, for nuclei) and Fluoro Nissl Blue (for MNs) RE and NH-treated (RE + NH) animals at 6 months post RE. (B) Top, Microphotographs showing co-localization of GAP43 (purple) or ChAT (red) with neurofilament NF200 (NF, green) at mid-level of sciatic nerves obtained from controlateral (CTL), RE, and RE + NH groups at 6 months post RE. Bottom, Bar graphs showing the average (±SEM) of the number of immunohistochemically detected GAP43⁺ (left) and ChAT⁺ (right) fibers at midlevel of sciatic nerve. Dotted lines indicate control value means for GAP43 and ChAT, respectively (n = 3 for CTL group and n = 4 for RE and RE + NH; t-test, *p < 0.05 RE + NH vs. RE). Scale bar = 100 µm for A and for GAP43/NF in B, and 25 µm for ChAT/NF in B.