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. 2017 Sep 7;170(6):1149–1163.e12. doi: 10.1016/j.cell.2017.07.028

Figure 1.

Figure 1

Distinct Mesenchymal Lineages Expressing Lgr5 and Lgr6 in Adult Lungs

(A and B) Representative confocal images showing expression patterns of Lgr6 in adult distal lungs: GFP (green); α-SMA (yellow); and DAPI (blue) in lung tissue sections from Lgr6-EGFP-CreERT2 mice. Arrowheads indicate vascular smooth muscle cells expressing α-SMA+. aw, airway; v, blood vessel.

(C) Representative profile of FACS-sorted EGFP+ populations from Lgr6-EGFP-CreERT2 mice for qPCR analysis.

(D) Validation of differential expression of Lgr5, Lgr6, and Acta2 in Lgr6+ and Lgr6− cells by qPCR analysis. Expression from Lgr6+ cells is shown as fold change relative to Lgr6− cells set to 1, followed by normalization to Gapdh.

(E and F) Representative confocal images showing expression patterns of Lgr5 in adult distal lungs: Tdtomato (red); α-SMA (yellow); and DAPI (blue) in lung tissue sections from Lgr5-CreERT2;R26-Tom mice, followed by Tamoxifen injection. aw, airway; v, blood vessel.

(G) Representative profile of FACS-sorted TdTomato+ populations from Lgr5-CreERT2;R26-Tom mice for qPCR analysis. Sorting scheme is same as in (C).

(H) Validation of differential expression of Lgr5, Lgr6, and Acta2 in Lgr5+ and Lgr5− cells by qPCR analysis. Normalized as in (D).

The scale bars represent 100 μm. Data presented are the mean of three independent experiments with triplicates. Error bars indicate SD (∗p < 0.001). See also Figure S1.