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. 2017 Sep 7;170(6):1149–1163.e12. doi: 10.1016/j.cell.2017.07.028

Figure 3.

Figure 3

Proliferative Potential of Lineage-Labeled Lgr6+ Cells in Homeostatic Lungs

(A and E) Schematics of the Lgr6-EGFP-CreERT2;R26-Tom lineage-tracing experiment.

(B and F) Representative flow cytometry analysis showing the recombination efficiency of lineage-labeled Lgr6+ cells after Tmx induction.

(C) Representative confocal images showing lineage-labeled Lgr6+ cells in adult lungs at 10 days and 12 months after Tmx induction: Tdtomato (red); α-SMA (yellow); and DAPI (blue).

(D) Graphs to show the mean percentage of lineage-labeled Lgr6+ airway smooth muscle cells (ASMCs) following Tmx exposure (red circles). Control animals receive vehicle alone, and low recombination is seen without Tmx injection (black circles).

(G) Representative confocal images showing proliferative expansions of lineage-labeled Lgr6+ cells in lung homeostasis: Tdtomato (red); α-SMA (yellow); and DAPI (blue).

(H and I) Size distribution of clusters at indicated time points post-induction. Length of bar represents frequency in (H).

n, number of clones scored. The scale bars represent 100 μm (C), 10 μm (G, top), and 50 μm (G, bottom).