Figure S2.
Heterogeneity of Lgr5- and Lgr6-Expressing Cells, Related to Figure 2
(A) Expression of Lgr5, Lgr6, and Acta2 in individual cells analyzed by single cell RNA-seq. Shown are boxplots of the distribution of expression levels (log2(TPM+1)) for Lgr5 (left panel), Lgr6 (middle panel), and Acta2 (right panel) in Lgr5+ (left bar) and Lgr6+ (right bar) cells (x axis).
(B) T-Stochastic neighbor embedding (tSNE) plot of 182 individual Lgr5+ (red and green, two replicates) and Lgr6+ (blue and purple, two replicates) cells, after quality filtering.
(C–G) tSNE plots as in (B) but where cells are colored by the expression of specific genes, as marked.
(H and I) Representative confocal images showing expression of Lgr5 and Lgr6 in airway epithelial cells in adult distal lungs: Tdtomato (for Lgr5 or Lgr6, red), CCSP (cyan), and DAPI (blue) in lung tissue sections from Lgr5-CreERT2;R26-Tom (H) and Lgr6-CreERT2;R26-Tom (I) mice, following by Tamoxifen injection (Tmx, 0.2mg/g x 4). Scale bars, low magnification 200um; high magnification 50um.