Fig. 1.

Imaging pipeline for three formalin fixed brain specimens to study fibre orientation dispersion. dMRI data were collected with 120 directions at 9.4 T, to which a parametric dispersion model was fit. After scanning, samples were cut coronally into two slabs that were processed separately to obtain fibre orientations at microscopic level. For one slab, PLI data were collected from serial sections, spanning ~1 mm in the cutting direction. The other part of the sample was immunohistochemically stained for proteo-lipid protein (PLP) as a measure of myelin content and for glial fibrillary acidic protein (GFAP) as a measure for astrocytes.