Table 1. Whole genome sequencing data for evolved P. aeruginosa populations.

Sample name	Gene name in PA14	Functional category	Mutation type	InDel (nts)	Mutation	Mutationa tolerated	Relative abundance in population (%)
M15A	wspA	Cell signaling	Del	Δ84	S285S	No	100
	lasR	Transcription	Del	Δ17	X17	—	40
M15B	wspA	Cell signaling	SNP	—	A438V	No	100
	lasR	Transcription	SNP	—	T222I	No	30
M15C	wspA	Cell signaling	SNP	—	A418V	No	100
	lasR	Transcription	SNP	—	C188R	No	40
C15A	wspF	Cell signaling	SNP	—	M307V	No	92
	orfN	LPS biosynthesis	In	+1	X99	—	45
	orfN	LPS biosynthesis	In	+2	X4	—	< 1
	dnpA	LPS biosynthesis	Del	Δ20	X2	—	4
	mexI	Defense mechanisms	SNP	—	G195E	No	< 1
C15B	PA14_65570	Unknown	Del	Δ9	L173A	No	59
	wspE	Cell signaling	SNP	—	A749V	No	33
	orfN	LPS biosynthesis	Del	Δ1	X7	—	52
	pilT	Cell signaling	Del	Δ1365	Gene deleted	No	50
	PA14_05160	Unknown	Del	Δ21	Δ7aa	—	50
C15C	wspF	Cell signaling	Del	Δ41	X129	—	30
	PA14_23400	Unknown	Del	Δ1	X22	—	41
	orfN	LPS biosynthesis	Del	Δ1	X7	—	16
	PA14_23400	Unknown	SNP	—	Q329K	No	24
	orfN	LPS biosynthesis	SNP	—	D249G	No	25
	wspF	Cell signaling	SNP	—	A160A	Yes	24
	dnpA	LPS biosynthesis	Del	Δ20	X2	—	2

Abbreviations: Δ, deletion of nucleotides; +, insertion of nucleotides; fs, frameshift; LPS, lipopolysaccharide; SNP, single nucleotide polymorphism; X, stop codon.

Co-culture samples from the 15th day are designated as C15, monoculture samples from the 15th day as M15.

^aas predicted by the SIFT algorithm (http://sift.jcvi.org/).