Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 20;85(10):e00305-17. doi: 10.1128/IAI.00305-17

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Lavender et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

PMC Copyright notice

FIG 2 — N. cinerea V1.110 fHbp binds CFH at high affinity. (A) Recombinant V1.110 fHbp from N. cinerea (N c) CCUG 346T, with V1.1 fHbp, and V1.1^I311A fHbp from N. meningitidis (N m) were recognized by Western blotting using polyclonal anti-V1.1 fHbp sera. N. cinerea V1.110 fHbp and V1.1 fHbp bound CFH by far-Western analysis with NHS as the source of CFH. Molecular masses are shown, and Coomassie blue staining of proteins is shown as the loading control. (B) SPR analysis of N. cinerea fHbp binding to recombinant CFH_6–7 (concentrations are indicated) with 1:1 Langmuir fit (black lines). (C) Calculated K_D values for CFH_6–7 binding to N. cinerea V1.110 fHbp and V1.1 fHbp; NBD, no binding detected. (D) ELISA of N. cinerea fHbp, V1.1 fHbp, and V1.1^I311A fHbp binding to full-length CFH. (E) Significance of CFH binding by ELISA analyzed at 5 μg/ml of CFH. The error bars indicate the standard error of the mean (SEM) from three independent experiments, and P values (***, P < 0.001; ns, P > 0.05) were calculated using a two-tailed unpaired t test.