TABLE 1.
Primary cells, cell lines, and culture conditions used in this studya
| Cell/cell line | Type/origin (source/reference) | Culture medium (source) | Bacterium/MOI/time p.i. (h) | No. of experiments/ no. of replicates |
Range of MOIs tested for MNGC formation |
|---|---|---|---|---|---|
| HGF | Fibroblast/gingiva (CLS) | FGM-MG (CLS) | B. pseudomallei/200/24 | 2/2 | 50–500 |
| hMSC | Stem cell/bone marrow (ATCC) | Mesenchymal stem cell medium (ATCC) | B. pseudomallei/100/23 | 1/2 | 10–400 |
| HUVEC | Endothelial/vascular (Lonza) | EGM-2 (Lonza) | B. pseudomallei/1/21 | 2/1 or 2 | NA |
| KC | Keratinocyte/epidermal foreskin (healthy volunteers) | KGM Gold, KGM-2 BulletKit (Lonza) | B. pseudomallei/50/21 | 2/3 | 50–200 |
| MDM | Peripheral blood mononuclear cells/blood (healthy volunteers) | RPMI 1640 (Biochrom), 10% FBS (Sigma) | B. pseudomallei/10/20 | 2/2 | 1–50 |
| NHBE | Epithelial/airways above bifurcation of the lungs (Lonza) | BEGM (Lonza) | B. pseudomallei/1/19 | 3/3 | NA |
| PMN | Granulocyte/blood (healthy volunteers) | RPMI 1640 (Biochrom), 10% FBS (Sigma) | B. pseudomallei/10/17 | 2/3 | 1–50 |
| 5637 | Carcinoma epithelial/bladder (CLS/51) | RPMI 1640 (Biochrom), 10% FBS (Sigma) | B. thailandensis/10/40 | 3/3 | 10–100b |
| DU-145 | Epithelial/brain derived metastatic prostate cancer (CLS/52) | DMEM (Gibco), 10% FBS (Sigma) | B. thailandensis/100/26 | 2/3 | 10–100b |
| HuTu-80 | Adenocarcinoma epithelial/duodenum (CLS/53) | DMEM (Gibco), 10% FBS (Sigma), 1% NEA (Biochrom) | B. thailandensis/50/18 | 2/3 | 10–100b |
| PANC-1 | Carcinoma epithelial/pancreas (CLS/54) | DMEM (Gibco), 10% FBS (Sigma) | B. thailandensis/100/14 | 3/3 | 10–100b |
| SK-OV-3 | Adenocarcinoma epithelial/ovary (CLS/51) | McCoy 5A (Biochrom), 2 mM l-glutamine (Gibco), 1× NEA (Gibco), 5% FBS (Sigma) | B. thailandensis/100/25 | 2/3 | 10–100b |
| SW13 | Carcinoma epithelial/adrenal gland (CLS/55) | 1:1 DMEM/Ham's F-12 (Gibco), 2 mM l-glutamine (Gibco), 5% FBS (Sigma) | B. thailandensis/50/20 | 3/3 | 10–100b |
a
Abbreviations: ATCC, American Type Culture Collection (www.atcc.org); CLS, Cell lines services (http://www.clsgmbh.de); DMEM, Dulbecco's modified Eagle medium; DSMZ, German Collection of Microorganisms and Cell Cultures (https://www.dsmz.de/); MDM, monocyte-derived macrophages; NEA, nonessential amino acids; NA, not applicable; p.i., postinfection.
b
MNGCs were observed at all MOIs used.