FIG 3.

PgLPS₁₆₉₀ inhibits hBD3- and LL-37-induced Ca²⁺ mobilization in LAD2 cells, but PgLPS_1435/1449 does not. (A and D) Indo-1-loaded cells were sequentially exposed to hBD3 (100 nM) or LL-37 (1 μM) and C3a (10 nM), and intracellular Ca²⁺ mobilization was determined. (B and E) Cells were pretreated with PgLPS₁₆₉₀ (10 μg/ml) before exposure to hBD3 (100 nM) or LL-37 (1 μM) and C3a (10 nM), and intracellular Ca²⁺ mobilization was determined. (C and F) Cells were pretreated with PgLPS_1435/1449 (10 μg/ml) before exposure to hBD3 (100 nM) or LL-37 (1 μM) and C3a (10 nM), and intracellular Ca²⁺ mobilization was determined. Traces are representative of three independent experiments.