TABLE 2.
Efficiencies of invasion of P. falciparum laboratory-adapted strains and a Senegalese culture-adapted isolate into knockdown cultured erythrocytes
| Strain | % efficiency of invasiona into erythrocyte type: |
||
|---|---|---|---|
| GPA KD | GPB KD | GPC KD | |
| Dd2 | 72.9 ± 18.3* | 56.4 ± 24.2** | 91.8 ± 8.2 |
| 3D7 | 90.2 ± 11.4 | 66.6 ± 23.3*** | 89.6 ± 9.8 |
| 3D7ΔEBA175 | 100.4 ± 31.8 | 53.4 ± 30.0 | 94.1 ± 16.5 |
| 3D7ΔRh2b | 101.2 ± 14.8 | 60.5 ± 26.6* | 100.2 ± 4.8 |
| 7G8 | 85.0 ± 31.5 | 56.1 ± 26.8 | 98.3 ± 21.9 |
| HB3 | 101.3 ± 43.7 | 53.6 ± 18.1 | 93.0 ± 15.5 |
| Sen51 | 109.7 ± 28.3 | 58.8 ± 16.8* | 113.0 ± 3.6 |
a
Efficiency of invasion into KD cells relative to pLKO control cells, based on final parasitemia. Parasitemia was determined by microscopy from counts of 500 to 2,000 erythrocytes, depending on the experiment. Invasion assays were performed in triplicate, four to six times for 3D7, Dd2, 7G8, and HB3 and three times for 3D7ΔEBA-175, 3D7ΔRh2b, and Sen51. Shown are the means and standard deviations. Statistical significance was determined using a one-way ANOVA with a Dunnett multiple-comparison test (*, P < 0.05; **, P ≤ 0.01; and ***, P ≤ 0.001).