FIG 3.

Tpl2 regulates claudin expression during C. rodentium infection. (A) Wild-type and Tpl2^−/− mice were gavaged with 2 × 10⁹ CFU Citrobacter rodentium (ICC180). Mice were euthanized at 8 or 11 dpi. The relative levels of expression of claudin 2 (Cldn2) and claudin 5 (Cldn5) in the colon were measured. Data from two or more independent experiments per time point were pooled (n ≥ 8 mice). *, P < 0.05 by two-way ANOVA. (B) Wild-type and Tpl2^−/− mice were gavaged with 2 × 10⁹ CFU of Citrobacter rodentium (ICC180) followed by FITC-dextran at 8 dpi. The concentrations of FITC-dextran in serum were quantified at 4 h posttreatment. Outliers were excluded from analysis using Grubb's test. **, P < 0.005 compared to control mice using one-way ANOVA.