FIG 5.
The neutrophil response to P. aeruginosa infection is regulated by 17β-estradiol. (A) Percentage and number of absolute neutrophils from whole lungs of C57BL/6 mice 9 h after P. aeruginosa infection with 3 ×105 CFU, quantified by flow cytometry (n = 6 mice per group per time point). (B) Percentage and absolute neutrophil counts from bronchoalveolar lavage fluid, quantified by flow cytometry (n = 6 per group per time point). (C) Kaplan-Meier survival plots of C57BL/6 ovariectomized and hormone-treated female mice rendered neutropenic by using the 1A8 monoclonal antibody (anti-Ly6G antibody). Ovariectomized mice were infected with 8 × 104 CFU of P. aeruginosa (n = 8 per group). (D) Neutrophils were isolated from mice and used ex vivo for P. aeruginosa killing experiments at an MOI of 1:4 under the indicated treatment conditions (n = 5 per treatment group). Data in panels A, B, D, and E are mean results ± SEM. *, P < 0.05 compared to vehicle.