FIG 7.

17β-Estradiol impacts responses to P. aeruginosa infection of neutrophils from subjects with CF. (A) Neutrophils from female subjects with CF treated ex vivo with E2 and ICI 182,780 (ICI) at the indicated doses were subjected to P. aeruginosa killing assays at an MOI of 1:4 (n = 20 per group). (B) Neutrophils from male subjects with CF treated ex vivo with E2 and ICI 182,780 (ICI) at the indicated doses were analyzed in P. aeruginosa killing assays at an MOI of 1:4 (n = 5). Data shown are mean results ± SEM. *, P < 0.05 relative to vehicle. (C) Neutrophils similar to those desribed for panel A were treated with E2 and ICI 182,780 for oxidative burst assays induced by P. aeruginosa. Chemiluminescence (CLU) is expressed as the mean result ± SEM (n = 6 per group). *, P < 0.05 (AUC). (D) NE expression in CF neutrophils treated with vehicle or E2. Data shown are the mean results ± SEM (n = 6). *, P < 0.05. (E) Neutrophil death was assayed via LDH release at the indicated time points (n = 6 per group). *, P < 0.05 relative to vehicle with PAO1 and vehicle without PAO1. Shown in panels A, C, and D are the mean results ± SEM. *, P < 0.05 relative to vehicle.