FIG 2.

(A) Relative reactive oxygen species (ROS) levels in rat peritoneal cells cultured for 16 h in vitro with (+) or without (−) T. gondii infection. Fluorescence generated by the reaction of CellROX Deep Red reagent (Molecular Probes) with cellular ROS is shown on the y axis as the mean fluorescence intensity (measured per microscopic field at a magnification of ×20) proportional to the level of ROS in the cells. Lew− and Lew+ represent LEW rat peritoneal cells without and with T. gondii infection, respectively. BN− and BN+ represent Brown Norway rat peritoneal cells without and with T. gondii infection, respectively. (B) Mean percentage of T. gondii-infected cells (black bars) and uninfected cells (gray bars) in cultures of LEW rat (LEW Cells) and BN rat (BN Cells) peritoneal cells cultured with T. gondii tachyzoites at an MOI of 1:1 for 16 h. (C) Representative images depict T. gondii-infected and uninfected LEW and BN peritoneal cells stained with CellROX Deep Red Reagent for detecting reactive oxygen species (red fluorescence) content in cells, captured at a magnification of ×20 at 16 h postinfection. (D) Representative images of LEW and BN peritoneal cells depicting the proportion of infected (with parasites fluorescing green) and uninfected cells at 16 h postinfection. The data shown represent means from three independent experiments with standard error bars, and the points at which data are significantly different (P < 0.05) are shown by asterisks.